expression and purification of recombinant outer surface protein d of borrelia burgdorferi

نویسندگان

m.h. sanati

f. alasti

h. aleyasin

m. mostafavi

p.r. carnegie

چکیده

to carry out the immunological experiments on the serum of multiple sclerosis (ms) patients, based on a correlation between borrelia burgdorferi infection and contracting ms autoimmune disease the outer surface protein d (ospd) of the bacterium was expressed and purified. a clone containing the ospd gene in pet11a expression vector under the control of t7 promoter was transformed to the bacterial host bl21 (de3). some of the colonies were selected for iptg-induced expression. the colony with the highest amount of ospd was selected for large-scale expression. large-scale protein purification was performed by the reversed phase hplc with a c4 preparative column; ultimately, the expressed purified protein was confirmed by the western blot technique.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Expression and Purification of Recombinant Outer Surface Protein D of Borrelia burgdorferi

To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacteri...

متن کامل

Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato.

The aim of this work was isolation and purification of the major immunodominant protein, Outer surface protein C (OspC) of three members of the species group Borrelia burgdorferi, the causative agent of Lyme disease. Our aim was to obtain this protein in a quantity and purity sufficient for immunization of experimental animals. For optimalization of protein purification's yield we used immobili...

متن کامل

Preparation and purification of recombinant outer surface protein A (rOspA) of Borrelia burgdorferi sensu stricto and Borrelia afzelii.

The recombinant Outer surface protein A (rOspA) from Borrelia burgdorferi is a possible immunogen for protection of infected humans and animals against development of Lyme borreliosis (Lyme disease), a chronic tick-borne disease characterised by diverse dermatologic, neurologic, rheumatic, and cardiac manifestations. For several years, research and development have been directed towards a vacci...

متن کامل

Identification of Two Epitopes on the Outer Surface Protein A of the Lyme Disease Spirochete Borrelia burgdorferi

A murine IgM monoclonal antibody (MA-2C6) with κ-light chains directed against an antigenic determinant of outer surface protein A (OspA) of the Lyme disease spirochete, Borreliaburgdorferi, is produced. This antibody could bind specifically to OspA antigen of several isolates of B. burgdorferi, but not to the non-Lyme disease bacteria such as T. pallidum and B. hermsii. Antibody MA-2C6 was pur...

متن کامل

Evolution of the Borrelia burgdorferi outer surface protein OspC.

The genes coding for outer surface protein OspC from 22 Borrelia burgdorferi strains isolated from patients with Lyme borreliosis were cloned and sequenced. For reference purposes, the 16S rRNA genes from 17 of these strains were sequenced after being cloned. The deduced OspC amino acid sequences were aligned with 12 published OspC sequences and revealed the presence of 48 conserved amino acids...

متن کامل

Role of outer surface protein D in the Borrelia burgdorferi life cycle.

Borrelia burgdorferi preferentially induces selected genes in mice or ticks, and studies suggest that ospD is down-regulated in response to host-specific signals. We now directly show that ospD expression is generally elevated within Ixodes scapularis compared with mice. We then assessed the importance of OspD throughout the spirochete life cycle by generating OspD-deficient B. burgdorferi and ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
archives of razi institute

ناشر: razi vaccine & serum research institute (rvsri)

ISSN 0365-3439

دوره 58

شماره 1 2016

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023